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Conquer Biology OST Practice Test

Ace your exam with curated practice questions

Difficulty: Moderate
Grade: Grade 10
Study OutcomesCheat Sheet
Colorful paper art depicting a trivia game about Biology OST Blitz for high school students.

What is the basic unit of life?
Atom
Cell
Organ
Molecule
Cells are the fundamental building blocks of all living organisms. They carry out essential functions necessary for life.
Which organelle is known as the powerhouse of the cell?
Nucleus
Mitochondria
Golgi apparatus
Chloroplast
Mitochondria generate most of the cell's ATP, serving as the primary energy source. This is why they are often referred to as the powerhouse of the cell.
What process do plants use to make their own food using sunlight?
Photosynthesis
Transpiration
Respiration
Fermentation
Photosynthesis allows plants to convert light energy into chemical energy stored in glucose. It is a fundamental process that supports life on Earth.
What is the main function of the cell membrane?
Produces energy
Synthesizes proteins
Stores genetic information
Controls what enters and leaves the cell
The cell membrane regulates the movement of substances into and out of the cell. This selective permeability is crucial for maintaining a stable internal environment.
Which macromolecule carries genetic information in most living organisms?
Carbohydrates
Lipids
DNA
Proteins
DNA contains the genetic blueprint for the development and functioning of all living organisms. It is responsible for heredity and guides protein synthesis.
Which organelle is responsible for protein synthesis in eukaryotic cells?
Mitochondria
Ribosomes
Golgi apparatus
Endoplasmic reticulum
Ribosomes are the sites where proteins are synthesized by translating mRNA into polypeptide chains. They are essential for cell function and can be found free in the cytoplasm or attached to the rough ER.
What process involves the passive movement of water across a semipermeable membrane?
Endocytosis
Diffusion
Osmosis
Active transport
Osmosis is the passive movement of water from an area of lower solute concentration to an area of higher solute concentration. It occurs across a semipermeable membrane without the input of energy.
During which phase of mitosis do chromosomes align at the cell's center?
Prophase
Telophase
Anaphase
Metaphase
During metaphase, chromosomes line up along the metaphase plate at the center of the cell. This proper alignment is critical for ensuring equal distribution of chromosomes to daughter cells.
How does DNA replication occur?
Semi-conservatively
Randomly
Dispersively
Conservatively
DNA replication is a semi-conservative process, meaning each new DNA molecule includes one original strand and one newly synthesized strand. This mechanism maintains the integrity of genetic information between generations.
What is the primary role of chlorophyll in photosynthesis?
Produce oxygen
Fix carbon dioxide
Absorb light energy
Split water molecules
Chlorophyll is essential for absorbing sunlight, which is then used to drive the light-dependent reactions of photosynthesis. This absorbed energy is converted into chemical energy for the plant.
What percentage of energy is typically transferred from one trophic level to the next in an ecosystem?
25%
75%
50%
10%
Approximately 10% of the energy from one trophic level is passed on to the next, with most energy lost as heat. This concept is fundamental to understanding energy flow in ecological systems.
Which characteristic is true of prokaryotic cells?
They undergo mitosis
They possess membrane-bound organelles
They have a defined endoplasmic reticulum
They lack a nucleus
Prokaryotic cells do not have a nucleus or any membrane-bound organelles, distinguishing them from eukaryotic cells. This simplicity is a defining characteristic of prokaryotes.
Which molecule serves as the direct energy currency of the cell?
Glucose
DNA
ATP
NADH
ATP, or adenosine triphosphate, is the molecule that directly provides energy for cellular functions. Its hydrolysis releases energy necessary for various metabolic processes.
During cellular respiration, what is the final electron acceptor in the electron transport chain?
Water
Oxygen
Carbon dioxide
Nitrogen
Oxygen serves as the final electron acceptor in the electron transport chain, combining with electrons and protons to form water. This step is vital for efficient ATP production during aerobic respiration.
Which statement best describes the role of enzymes in biological reactions?
Enzymes lower the activation energy of reactions
Enzymes permanently alter substrates
Enzymes raise the activation energy needed
Enzymes are consumed during reactions
Enzymes act as catalysts by lowering the activation energy required for biochemical reactions, thereby speeding up the reaction rate. They are not consumed in the process, which allows them to be used repeatedly.
Which process results in the formation of genetic diversity during meiosis?
Mitosis
Binary fission
Crossing over
Budding
Crossing over during prophase I of meiosis facilitates the exchange of genetic material between homologous chromosomes. This process increases genetic diversity in the resulting gametes.
Which mechanism is most directly responsible for increasing the frequency of a beneficial allele in a population?
Mitosis
Mutation
Genetic drift
Natural selection
Natural selection favors individuals with beneficial alleles, thereby increasing their frequency in the population over time. This process drives adaptive evolution and improved fitness.
What is the main difference between aerobic and anaerobic respiration?
The presence of oxygen in aerobic respiration
The production of ATP
The occurrence in eukaryotes exclusively
The utilization of glucose
Aerobic respiration requires oxygen to efficiently produce ATP, whereas anaerobic respiration occurs without oxygen and yields less ATP. This difference is critical for understanding how cells adapt to varying oxygen conditions.
Which cell signaling mechanism involves the binding of a ligand to an intracellular receptor, usually affecting gene expression?
Paracrine signaling
Endocrine signaling
Autocrine signaling
Intracellular signaling
Intracellular signaling involves ligands that enter the cell and bind to internal receptors, directly influencing gene expression. This mechanism differs from extracellular signaling, where ligands interact with cell surface receptors.
How does speciation most commonly occur in geographically isolated populations?
Parapatric speciation
Allopatric speciation
Artificial selection
Sympatric speciation
Allopatric speciation occurs when populations are geographically separated, leading to reproductive isolation and genetic divergence. Over time, these isolated populations accumulate differences that result in the formation of new species.
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Study Outcomes

  1. Understand and recall key biological concepts swiftly.
  2. Analyze relationships among biological structures and processes.
  3. Apply rapid decision-making skills in exam scenarios.
  4. Evaluate biological data to draw evidence-based conclusions.
  5. Synthesize information to solve biology-related problems.

Biology OST Practice Test Cheat Sheet

  1. Cell Organelles: Nucleus, Mitochondria & Ribosomes - Dive into the microscopic world where each organelle has a superstar role. The nucleus holds the cell's blueprint, mitochondria generate ATP like tiny power plants, and ribosomes assemble proteins in a molecular factory line. Understanding these players is your ticket to mastering cell biology! Biology End‑of‑Course Study Guide
  2. studylib.net biology guide
  3. Stages of Cellular Respiration - Explore how cells harvest energy in three thrilling acts: glycolysis in the cytoplasm, the Krebs cycle in the mitochondrial matrix, and the electron transport chain across the inner membrane. Each step unlocks more ATP, fueling everything from muscle contractions to brainpower. Remember: respiration is like a cellular relay race handing off energy packets! Biology End‑of‑Course Study Guide
  4. studylib.net biology guide
  5. Photosynthesis: Light Reactions & the Calvin Cycle - Watch plants turn sunlight into sugar through two dynamic stages. Light reactions capture photons to make ATP and NADPH, while the Calvin cycle fixes CO₂ into glucose - a sweet success story! Try the mnemonic "Light makes loads of power, Calvin cycles sugar showers." Biology End‑of‑Course Study Guide
  6. studylib.net biology guide
  7. DNA Structure & Semi‑Conservative Replication - Picture a twisted ladder of nucleotides where A pairs with T and C pairs with G in a perfect dance. During replication, each strand serves as a template, ensuring genetic info is copied with high fidelity. It's like a molecular photocopier with built‑in proofreading! Biology End‑of‑Course Study Guide
  8. studylib.net biology guide
  9. Mendelian Genetics Essentials - Learn why dominant and recessive alleles determine traits and how homozygous versus heterozygous pairings predict offspring. Punnett squares are your visual cheat sheet - flip two Aa parents together for that classic 3:1 ratio of dominant to recessive traits. Genetics is just probability with a green thumb! Biology End‑of‑Course Study Guide
  10. studylib.net biology guide
  11. Evolution Mechanisms: Natural Selection & More - From survival of the fittest to random genetic drift, evolution shapes life's tapestry over time. Natural selection amplifies advantageous traits, while gene flow and drift add spice to population genetics. Think of it as nature's long‑term makeover show for species! AP Biology Key Concepts
  12. stemlytutoring.com AP Biology guide
  13. Levels of Ecological Organization - Zoom out from individual organisms to populations, communities, ecosystems, and the entire biosphere. Each level reveals new interactions, from friendly symbiosis to fierce competition. It's like nesting dolls of biology - every layer matters! Biology End‑of‑Course Study Guide
  14. studylib.net biology guide
  15. Macromolecules: Carbs, Lipids, Proteins & Nucleic Acids - These heavyweight champs power cells: carbs provide quick energy, lipids store fuel long‑term, proteins build structures and catalyze reactions, and nucleic acids carry genetic blueprints. Imagine a bustling city where each macromolecule has its own job! Biology End‑of‑Course Study Guide
  16. studylib.net biology guide
  17. The Cell Cycle: Interphase, Mitosis & Cytokinesis - Cells grow, duplicate DNA, and split in a precise sequence - G₝, S, G₂ during interphase, then PMAT for mitosis, and finally cytokinesis. Use the "PMAT" trick to ace mitosis stages: Prophase, Metaphase, Anaphase, Telophase. It's cellular choreography at its finest! Biology End‑of‑Course Study Guide
  18. studylib.net biology guide
  19. Biotechnology Basics: PCR, Cloning & Gel Electrophoresis - PCR is like a molecular photocopier, cloning copies DNA segments, and gel electrophoresis separates fragments by size in a genetic obstacle course. These tools power breakthroughs in medicine, forensics, and research. Get ready to be the next biotech whiz! Biology End‑of‑Course Study Guide
  20. studylib.net biology guide
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