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Quizzes > Health & Medicine > Human Anatomy & Medicine

Master Donning Sterile Gloves: Take the Quiz Now

Ready to prove your sterile glove donning skills? Start the free test!

Difficulty: Moderate
2-5mins
Learning OutcomesCheat Sheet
Paper art illustration of gloved hands and aseptic field checklist on teal background for sterile gloves quiz

Ready to master donning sterile gloves? Our Donning Sterile Gloves Quiz: Test Your Aseptic Skills welcomes clinicians, OR teams, and sterile processing techs to challenge their techniques. This free, timed test dives into each critical step of aseptic gloving technique and highlights best practices in the donning of sterile gloves. You'll learn how to maintain a contamination-free field, pinpoint knowledge gaps in surgical glove donning, and gain confidence for any procedure. Jump in now to hone your skills, explore our skills assessment and grab expert interview prep tips to stand out in sterile processing. Take the quiz today and set a new standard in aseptic care!

What is the first action a healthcare professional should take before donning sterile gloves?
Perform hand hygiene
Inspect the surgical instrument
Put on the face mask
Adjust the sterile field
Proper hand hygiene is the foundational step in aseptic technique, reducing transient flora and preventing contamination of sterile gloves. Skipping hand hygiene increases the risk of surgical site infections. This is emphasized in the CDC’s hand hygiene guidance. CDC Hand Hygiene Guidelines
Which surface of a sterile glove is considered sterile?
Outer surface
Inner surface
Cuff interior
Packaging surface
After donning, the outside of the glove remains in the sterile field while the inside contacts skin and is thus considered non-sterile. The cuff interior also becomes contaminated when touched. AORN standards explain these distinctions. AORN Guidelines for Sterile Technique
How should the sterile glove package be opened to maintain sterility?
Pull the top flap away from you
Push the top flap towards you
Tear the package on the sides
Lift the package by its corners
Opening the package by pulling the top flap away prevents the gloves from brushing against unsterile surfaces. Pushing toward yourself risks contamination. The Joint Commission outlines proper package opening techniques. The Joint Commission
When picking up the first glove with your bare hand, which part should you touch?
The folded cuff of the glove
The palm of the glove
The tips of the fingers
The outer surface near the fingers
Touching only the folded cuff of the first glove ensures you avoid contact with the sterile outer surface. This preserves sterility until the glove is properly positioned. WHO infection prevention guidelines cover this step. WHO Infection Prevention Guidelines
Which hand should be gloved first in standard sterile glove donning?
Dominant hand
Non-dominant hand
Left hand
Right hand
Gloving the dominant hand first allows the more dexterous hand to manage the first glove, making the process smoother and safer. This is a widely taught convention in aseptic technique. Association for the Advancement of Medical Instrumentation
If a glove tears during donning, what is the correct action?
Discard that glove and start over
Patch the tear with tape
Continue with the coated glove
Use tape to secure the tear
A tear compromises the sterile barrier and must be immediately replaced to prevent contamination. Repairing with tape is not acceptable in sterile technique. CDC HICPAC guidelines mandate regloving. CDC HICPAC Guidelines
Why must hands be kept above waist level after donning sterile gloves?
To keep them within the visual field and maintain sterility
To reduce hand fatigue
To improve dexterity
To keep them close to the surgical field
Keeping hands above waist level and in view reduces the risk of accidental contact with unsterile surfaces. Below waist level is considered unsterile. WHO guidance emphasizes this position. WHO Infection Prevention
What is the proper hand position after sterile gloves are on, before beginning a procedure?
Hands held above waist with fingers pointed upward
Hands resting on the sterile field
Hands clasped at chest level
Hands crossed in front of body
Holding hands above waist with fingers upward ensures they remain in the sterile zone and visible to all team members. Any other position risks contamination. AORN details this standard. AORN Guidelines
What is the correct sequence for applying a sterile gown and gloves?
Put on gown, then gloves
Put on gloves, then gown
Perform hand hygiene after gowning
Put on mask before gloves
Standard practice is to don the sterile gown first, then apply gloves using the open or closed technique. Gloving before gowning can contaminate the gown. The Joint Commission provides this sequence. The Joint Commission
What is the purpose of the closed gloving technique?
To maintain sterility by keeping hands inside gown sleeves during gloving
To glove without a gown
To reduce time in preparation
To allow glove change during procedure
Closed gloving uses gown sleeves as a barrier so hands never exit the sterile gown, protecting both the hands and the gown cuff. This reduces contamination risk. AAMI standards describe this method. AAMI Standards
How far above the sterile field should you hold items when adding them to maintain sterility?
At least 6 inches (15 cm) above the field
At waist level
At eye level
Directly on the field
Holding items at least 6 inches above prevents accidental contact with the sterile surface. Anything held lower may fall onto the field. CDC infection control guidelines specify this distance. CDC Infection Control
Which action indicates a breach in a sterile field during glove application?
Touching the inside of the glove with bare skin
Sliding the hand directly into the glove
Keeping hands above the waist
Using open technique
Contact between ungloved skin and the glove’s interior or exterior constitutes contamination. Any such breach requires regloving. WHO aseptic technique guidelines clarify this point. WHO Guidelines
When donning the second glove, which method ensures sterility?
Slip fingers under the cuff of the first glove to grasp the second
Use bare fingertips on the second glove cuff
Grab the outside surface
Pinch the cuff from the outside
Using the sterile first glove cuff to handle the second glove’s interior maintains a sterile chain. Touching the outside of the second glove with bare skin is not allowed. AORN describes this technique. AORN Guidelines
What should you do if the glove cuff rolls down after application?
Remove both gloves and repeat donning
Roll the cuff back manually
Continue and adjust later
Use sterile forceps to fix
A rolled cuff exposes non-sterile skin and must be corrected by removing all gloves and starting over. Manual rolling contaminates the glove interior. CDC HICPAC supports this action. CDC HICPAC Guidelines
How should sterile gloves fit to ensure proper function?
Snugly, without excessive wrinkling or tightness
Loosely with room to move
With a one-inch gap at the fingertips
Tightly until circulation is reduced
A proper fit minimizes hand fatigue and risk of tear, while avoiding constriction. Wrinkles or gaps compromise function and increase contamination risk. AAMI glove sizing standards explain this. AAMI Standards
What should you check on a glove package to verify its sterility before use?
Expiration date and sterilization indicator
Manufacturer name and color
Package weight and size
Sterile field indicator
Verifying an unexpired date and a properly changed sterilization indicator ensures the gloves have been subjected to correct sterilization processes. Other package details do not confirm sterility. The Joint Commission outlines these checks. The Joint Commission
What temperature range is generally recommended for storing sterile gloves?
20-25°C (68-77°F)
0-5°C
37-40°C
10-15°C
Storing gloves at room temperature (20-25°C) preserves material integrity and sterility. Extremes of temperature can degrade the glove material or compromise the barrier. AAMI storage guidelines provide these ranges. AAMI Standards
Why must gloved hands be kept in view of the wearer at all times during a procedure?
To ensure they do not become contaminated
To improve comfort
To warm the gloves
To maintain blood flow
Keeping gloved hands in sight allows immediate recognition of breaches or contact with non-sterile zones. Out of view increases the risk of unrecognized contamination. AORN sterile field standards require constant visibility. AORN Guidelines
If a gloved hand inadvertently dips below the sterile field level, what is the immediate action?
Discard gloves and reglove
Wipe and continue
Raise hand back into field
Ask assistant to adjust
Allowing hands below waist level constitutes contamination. The only safe corrective is to remove the gloves and don a new pair to restore asepsis. CDC HICPAC details this protocol. CDC HICPAC Guidelines
What is the primary reason for using powder-free gloves in sterile procedures?
To reduce particulate contamination
To improve grip
To lower cost
To enhance comfort
Powder can carry particulates and allergens into the surgical site, increasing infection risk. Powder-free gloves eliminate this contamination source. FDA guidelines support powder-free use in sterile settings. FDA Guidelines
During surgery, you notice a small puncture in your sterile glove. What is the best practice?
Remove and reglove immediately
Cover with sterile tape
Ignore and continue
Sterilize in place
A puncture compromises the sterile barrier by allowing microbial entry. The correct approach is to stop, remove both gloves, and don a fresh pair. NIOSH guidelines mandate immediate regloving. NIOSH Guidelines
What action should be taken if the outside of the glove cuff becomes wet?
Remove and reglove
Wipe with sterile towel
Roll cuff back
Continue as normal
Moisture on the glove cuff can wick contaminants into the sterile glove interior. The only safe remediation is to discard the gloves and don a new pair. WHO infection prevention guidance covers this scenario. WHO Infection Prevention
In open gloving, why is it essential to grasp the glove at the inner cuff when donning the second glove?
To avoid contact with the glove’s outside surface and maintain sterility
To speed up the process
To secure a tighter fit
To avoid touching the gown
Grasping the inner cuff of the second glove with the sterile first glove prevents touching the outer surface, preserving sterility. This ensures a proper barrier between the glove exterior and the hands. AORN open gloving guidelines explain this detail. AORN Guidelines
Which microbiological advantage does the closed gloving technique provide over the open technique?
It shields the hands within gown sleeves, minimizing exposure to contaminants
It speeds up application time
It uses fewer gloves
It increases tactile sensation
Closed gloving keeps hands inside the sterile gown sleeves during gloving, reducing the chance of contact with non-sterile surfaces. This barrier method is microbiologically superior in high-risk procedures. AAMI standards describe this advantage. AAMI Standards
How can glove material fluorescence under ultraviolet light improve surgical safety?
By revealing micro?punctures and breaches in the glove barrier
By sterilizing the gloves
By warming the gloves
By changing color during surgery
Fluorescent markers in glove material show up under UV light, helping detect tiny punctures that are invisible to the naked eye. This early detection prevents unnoticed contamination during critical procedures. FDA surgical glove standards discuss this feature. FDA Surgical Glove Standards
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Study Outcomes

  1. Understand Aseptic Principles -

    Learn the fundamental concepts of aseptic technique and why maintaining sterility is vital during surgical glove donning and other clinical procedures.

  2. Identify Key Steps of Donning Sterile Gloves -

    Recognize the correct order of motions and preparations required for donning sterile gloves, from hand hygiene to final glove adjustment.

  3. Demonstrate Correct Sterile Glove Donning Technique -

    Apply the precise movements and handling methods necessary to perform sterile glove donning without contaminating the gloves or sterile field.

  4. Apply Aseptic Gloving Technique in Practice -

    Use best practices for surgical glove donning in lab or clinical scenarios, ensuring consistent sterility and confidence in every procedure.

  5. Evaluate and Correct Common Mistakes -

    Analyze potential errors in donning sterile gloves and learn strategies to troubleshoot and prevent contamination during glove application.

Cheat Sheet

  1. Hand Hygiene & Glove Selection -

    Start with meticulous handwashing following CDC guidelines, scrubbing for at least 20 seconds to remove transient flora. Choose the correct glove size to ensure snug fit without tears - Mnemonic: "Right Fit, Right Coverage" to remember that proper sizing is critical. This foundational step reduces microbial load and maximizes dexterity when performing sterile glove donning.

  2. Preparing the Sterile Field -

    Open the glove package slowly on a clean, flat surface, unfolding the top flap away from you to maintain sterility; side flaps come next, creating a "sterile pocket." Use the "Outside to You" mnemonic to recall that only the interior of the wrapper and gloves are sterile. Proper prep of the aseptic field prevents unintended contamination before glove application.

  3. Dominant Hand Gloving Technique -

    With your dominant hand, grasp the inner cuff of the first glove at the folded edge, taking care not to touch the outer surface. Lift the glove straight up and slide your hand in, stopping at wrist level. This scoop method preserves the sterile exterior and is recommended in surgical glove donning protocols by leading nursing schools.

  4. Non-Dominant Hand Gloving Technique -

    Using the newly gloved dominant hand, slip two fingers under the cuff of the second glove and pull it over the non-dominant hand without touching your skin. Keep hands above waist level and fingertips pointed up to prevent droop contamination. This aseptic gloving technique ensures both hands remain sterile and ready for clinical tasks.

  5. Maintaining Glove Integrity & Sterile Field -

    After donning, inspect gloves for tears or holes by flexing fingers and palms under bright light. Keep hands in the "sterile zone" above waist and in front of the body to avoid accidental contact with non-sterile surfaces. If a breach occurs, immediately discard and re-glove to uphold aseptic standards as outlined by infection-control research.

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