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Take the Laboratory Safety Knowledge Test

Test Your Lab Safety Proficiency in Minutes

Difficulty: Moderate
Questions: 20
Learning OutcomesStudy Material
Colorful paper art depicting elements of a Laboratory Safety Knowledge Test quiz

Ready to evaluate your lab safety skills? This laboratory safety quiz features 15 multiple-choice questions designed for students, educators, and lab professionals to sharpen safety awareness. Whether you're reviewing standard procedures or testing best practices, you'll gain confidence in identifying hazards and responding to emergencies. Feel free to customise this quiz in the quizzes editor - no coding required. For more focused practice, explore the Laboratory Compliance Assessment Quiz or the Fire Safety Knowledge Test.

Which piece of personal protective equipment primarily protects your eyes in a laboratory?
Lab coat
Safety goggles
Nitrile gloves
Respirator
Safety goggles are designed to protect the eyes from chemical splashes and debris by forming a tight seal around the eye area. Other PPE items do not provide direct eye protection.
Which fire extinguisher class is suitable for electrical fires?
Class A
Class D
Class C
Class B
Class C extinguishers are specifically rated for electrical fires because they use non-conductive agents to interrupt the electrical current. Using other classes on live equipment can be dangerous or ineffective.
What is the primary function of a chemical fume hood?
Measure solution pH
Sterilize laboratory equipment
Remove airborne hazardous vapors
Contain biological pathogens
A chemical fume hood uses controlled airflow to remove hazardous vapors and particulates away from the user. It protects both the laboratory worker and the environment by filtering or exhausting contaminated air.
Where should you dispose of broken glassware in a laboratory?
Regular trash
Chemical waste bin
Sharps disposal container
Autoclave bag
Broken glassware can cause cuts or puncture wounds and must be handled safely. Sharps disposal containers are designed to securely hold sharp items and prevent injury during waste handling.
The acronym SDS stands for which of the following?
Standard Disposal System
Safety Data Sheet
Safety Disposal Schedule
Secure Dewar Storage
SDS stands for Safety Data Sheet, which provides detailed information on chemical hazards and safe handling procedures. It is an essential reference for understanding risks and emergency measures related to a substance.
Which section of a Safety Data Sheet details recommended first-aid measures?
Section 16: Other information
Section 8: Exposure controls
Section 4: First-aid measures
Section 2: Hazard identification
Section 4 of an SDS provides detailed first-aid instructions for exposure scenarios such as inhalation, skin contact, and eye contact. This helps responders deal with emergencies safely and effectively.
When a chemical spill occurs, what is the first action to take?
Evacuate the building
Immediately attempt to neutralize the spill
Pour water over the spill
Consult the SDS and alert others
The SDS contains emergency procedures and hazard information necessary for safe spill response. Alerting others ensures that trained personnel can assist and contamination is contained quickly.
Which hazard pictogram indicates a flammable substance?
Health hazard silhouette
Skull and crossbones
Test tube pouring on hand (corrosion)
Flame symbol
The flame pictogram is used to indicate flammable gases, liquids, and solids. It warns users of fire and ignition risks associated with the substance.
What type of gloves is most suitable for handling strong acids?
Cotton gloves
Nitrile gloves
Latex gloves
Leather gloves
Nitrile gloves offer superior chemical resistance compared to latex or cotton and are suitable for handling strong acids. Using the correct glove material prevents permeation and skin exposure.
How should chemical waste containers be labeled?
With chemical identity and hazard warnings
No label is needed if sealed
With the container owner's name only
With disposal date only
Chemical waste containers must be labeled with the substance identity and associated hazards to ensure proper handling and disposal. Accurate labeling helps waste management staff apply the correct treatment processes.
Which is a biological hazard in the laboratory?
Bacterial culture spill
Overheated burner
Acid splash
Broken glass
Biological hazards include agents like bacteria, viruses, and other microorganisms that can cause disease. A bacterial culture spill poses an infection risk and must be managed with biohazard protocols.
Which of the following best describes a risk assessment?
Logging inventory levels
Identifying hazards and evaluating controls
Writing experimental protocol
Estimating disposal costs
Risk assessment involves identifying potential hazards, evaluating the likelihood and severity of harm, and implementing controls to reduce risk. It is a systematic process that guides safe laboratory practices.
What is the proper way to transport concentrated acids within the lab?
In an open beaker
In a paper bag
In a spill tray with a sealed, labeled container
Without label in hand
Transporting acids in sealed, labeled containers placed on a spill tray prevents leaks and informs others of the hazard. The tray confines any accidental drips or spills for safer movement around the lab.
Before beginning work with a new reagent, you should do what?
Test it by smelling
Mix it with water to dilute
Assume it's safe if unlabeled
Refer to its SDS for hazards
The SDS provides comprehensive hazard, handling, and storage information for a chemical reagent. Reviewing it before use ensures you understand any risks and necessary precautions.
What is the primary purpose of a biosafety cabinet?
Sterilizing biological samples
Containing chemical fumes
Protecting the user and environment from biohazards
Refrigerating samples
Biosafety cabinets use HEPA filtration to prevent exposure of the user and environment to infectious agents. They are essential when working with cell cultures, viruses, and other biohazardous materials.
A lab worker accidentally ingests a small amount of a corrosive chemical. According to standard emergency procedures, what should be performed next?
Drink large amounts of water or milk and seek medical attention
Induce vomiting immediately
Neutralize in the stomach with a base
Panic and leave the laboratory
Inducing vomiting after ingesting corrosive chemicals is contraindicated because it can cause further tissue damage. Diluting the substance with water or milk and seeking medical attention reduces harm.
You notice improper storage: incompatible chemicals stored together. Which combination is especially dangerous?
Sodium chloride and potassium nitrate
Hydrochloric acid and sodium hydroxide
Distilled water and ethanol
Magnesium sulfate and glucose
Mixing strong acids and bases can lead to violent neutralization reactions that generate heat and splashing. Storing them apart prevents accidental contact and dangerous exothermic reactions.
A spill of a flammable solvent occurs. Which fire extinguisher should be used?
Water extinguisher
Foam for protein fires
CO2 extinguisher
Dry powder for metal fires
CO2 extinguishers displace oxygen and are ideal for fires involving flammable liquids, avoiding spread or re-ignition. Water-based extinguishers can spread the solvent and worsen the fire.
Interpreting hazard statements on an SDS, what does "H350" indicate?
Causes severe skin burns
May cause cancer
Fatal if inhaled
Highly flammable liquid
The H350 code on an SDS indicates a carcinogenic hazard with the wording "May cause cancer." Hazard statements like H350 provide standardized information about specific risks.
During a risk assessment, which control measure hierarchy is most effective at the top?
Engineering controls
Elimination of hazard
Administrative controls
Personal protective equipment
The hierarchy of controls ranks elimination of the hazard as the most effective measure because it removes risk entirely. Other controls like PPE are less reliable since they depend on correct usage.
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Learning Outcomes

  1. Identify common chemical and biological hazards
  2. Apply proper use of personal protective equipment
  3. Demonstrate correct emergency response procedures
  4. Evaluate safe handling and disposal of lab waste
  5. Analyse risk factors and prevention strategies
  6. Master safety data sheet interpretation and use

Cheat Sheet

  1. Types of Chemical Hazards - Dive into the world of corrosives, flammables, and toxins to understand how each can harm you in the lab. By spotting these hazards early, you'll be ready to set up the right safety shields and keep experiments on track. Laboratories Overview | OSHA
  2. Identifying Biological Hazards - From mischievous bacteria to stealthy viruses and fungi, learn to recognize the tiny troublemakers that can cause infections. Proper containment and handling will turn you into a biosafety champion and keep your team healthy. Biological Hazards | OSHA eTool
  3. Using Personal Protective Equipment - Gloves, lab coats, and goggles are your personal force field against spills and splashes. Master the art of selecting, wearing, and disposing of PPE so nothing dangerous sneaks past your defenses. PPE Toolkit | OneLab REACH
  4. Emergency Response Procedures - Be the hero who knows exactly what to do when chaos strikes: spills, fires, or sudden accidents. Learn spill containment, evacuation routes, and first-aid steps so you can stay cool under pressure. Lab Safety Portal | CDC
  5. Laboratory Waste Management - Discover the do's and don'ts of disposing chemical, biological, and sharps waste without turning the lab into an eco-disaster zone. Proper segregation and labeling keep both you and the environment safe. Lab Safety Portal | CDC
  6. Risk Analysis and Prevention - Turn detective by analyzing each procedure for hidden hazards and apply strategies like fume hoods or SOPs to eliminate risks. A little foresight today means fewer surprises tomorrow! Laboratories Overview | OSHA
  7. Safety Data Sheet (SDS) Mastery - Crack open those SDS documents to uncover vital info on chemical properties, hazards, and safe handling tips. Knowing how to read them makes you the ultimate lab safety guru. OSHA 1910.1450 Standard
  8. Biosafety Levels - Learn the different BSLs from 1 to 4 and the strict practices and equipment each level demands. This knowledge ensures you're working at the right containment level for any infectious agent. Biosafety Guidelines | PMC
  9. Chemical Hygiene Plan (CHP) - Discover how a well-crafted CHP outlines essential procedures, equipment, and work practices to shield everyone from chemical risks. It's your lab's roadmap to safety compliance. OSHA 1910.1450 Standard
  10. Ongoing Safety Training - Stay ahead of the curve by exploring cutting-edge resources and guidelines from leading institutions. Continuous learning turns good safety habits into second nature. Lab Safety Training | NIH
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