Molecular Biology
Which of the following statement is false about DNA?
Located in chromosomes
There is a precise correlation between amount of DNA and number of sets of chromosome per cell
Carries genetic information from parent to offspring
Abundantly found in cytoplasm
What stores the genetic information in DNA?
Sugar
Polymerase
Nitrogenous base
Phosphate
All of the following regarding gel electrophoresis are true EXCEPT:
Agarose gel is used
Molecules move towards the positive electrode (d)
The higher the density of the gel, the higher the resolution
Smaller molecules move faster than larger ones
RNA molecules contain an additional oxygen atom compared to DNA molecules located on which carbon atom of the pentose sugar
1
2
3
4
5
Nitrogenous bases are attached to sugar molecules by:
hydrogen bonds
ionic bonds
glycosidic linkage
disulfide bridges
phosphodiester bonds
Each nucleotide is attached to the other nucleotide by :
ionic bonds
disulfide bridges
phosphodiester bonds
hydrogen bonds
glycosidic linkages
Nitrogenous bases are attached to each other by :
hydrogen bonds
ionic bonds
glycosidic linkages
phosphodiester bonds
What is the maximum number of phosphate groups that can be attached to pentose sugars in nucleotides
1
2
3
4
5
Denaturation of DNA molecules is a necessity in southern blotting in order to allow binding between the probs and the separated DNA strands , which of the following pH values allow this to occur and specifically in southern blotting
6.5
7
13
a+b
non of the above
Which of the following factors will contribute in the stabilization of DNA molecules :
a highly concentrated solution of urea
decreasing amounts of Na+ ions
greater number of G/C base pairs.
Which of the following diseases is primarily detected by RFLP ?
cystic fibrosis
sickle cell anemia
dementia
stromme syndrome
If there are 3 restriction sites, how many DNA fragments will be produced?
1
2
3
4
Nucleic acids are paired by Hydrogen bond
True
False
With respect to nucleosides which of the following is paired correctly?
Purine – Adenosine, Thymidine
Purine – Guanosine, Thymidine
Pyrimidine – Uridine, Cytidine
Pyrimidine – Uridine, Adenosine
Which of the following is not a component of the nucleic acid backbone?
Pentose sugar
Phosphodiesterase bond
Phosphate group
Nucleotide
In a diploid organism with 30,000 bases haploid genome contains 23% A residues. What is the number of G residues in the genome of this organism?
16000
14200
16500
16200
What should be the complementary strand of 3’….ATGGCTTGA….5’?
3’….TACCGAACT….5’
5’….TACCGAACT….3’
3’….TAGGCAAGT….5’
5’….TAGGCAAGT….3'
In a DNA double helix the bases are held together by hydrogen bonds. These hydrogen bonds are _________
Covalent bonds
Non-covalent bonds
Ionic bonds
Van der Waals forces
It is easy to break the bond between A and T than in between G and C.
True
False
Nucleosome is made up of __________
DNA, histone core protein
RNA, histone core protein, linker H1
RNA, histone core protein
DNA, histone core protein, linker H1
Histones have a high content of negatively charged amino acids.
TRUE
FALSE
Positioning of nucleosome is beneficial ___________
True
False
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