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Take the HIV Self-Testing Knowledge Quiz

Assess your understanding of at-home HIV testing

Difficulty: Moderate
Questions: 20
Learning OutcomesStudy Material
Colorful paper art depicting elements related to HIV self-testing knowledge quiz.

Looking to gauge your familiarity with self-administered HIV testing? This HIV Self-Testing Knowledge Quiz offers practical questions to test procedures, result interpretation, and safety steps. Ideal for health educators or individuals seeking a refresher before screening, this quiz builds confidence and clarity. For a broader challenge, try the HIV Knowledge Assessment Quiz or reflect insights with the Self Perspectives Assessment . All quizzes are fully editable in our intuitive editor.

What is the first action a user should take before performing an HIV self-test?
Use the swab right away.
Read all instructions carefully.
Eat a snack.
Clean test area with alcohol.
Reading instructions ensures the user understands each step, preventing errors and ensuring accurate results. It is crucial to familiarize oneself with the procedures before beginning.
Which specimen is commonly used in an oral fluid-based HIV self-test?
Blood from a finger prick.
Saliva collected from gums.
Urine sample.
Hair sample.
Oral fluid-based tests collect saliva from the gums or inner cheek to detect HIV antibodies. No blood draw is required, making the test less invasive.
After applying the sample and buffer, how long should you wait before reading the result of most HIV self-tests?
1 - 2 minutes.
15 - 20 minutes.
60 - 90 minutes.
Immediately.
Most HIV self-tests require a 15 - 20 minute development time for antibodies to appear. Reading too early or too late can lead to inaccurate results.
On an HIV self-test strip, what does the appearance of a control line indicate?
Test ran correctly.
Positive result.
Negative result.
Test is invalid.
A control line confirms that the sample and reagents flowed correctly through the test strip. Without a control line, the test cannot be considered valid.
How should you dispose of an HIV self-test kit after use?
Flush down the toilet.
In household trash without protection.
Place in sealed bag and dispose according to local guidelines.
Burn immediately.
Sealing the used kit in a bag and following local disposal guidelines prevents potential exposure to biohazardous materials. Proper disposal protects both the user and sanitation workers.
What is the 'window period' in HIV self-testing?
Time to read the result.
Time between infection and detectable markers.
The validity period of the test kit.
Time to confirm results.
The window period is the time after HIV infection when markers are not detectable by tests. Testing during this period may yield false-negative results.
If an HIV self-test result is positive, what is the recommended next step?
Repeat the self-test immediately.
Schedule a confirmatory test at a healthcare facility.
Assume positive and take no further action.
Start medications without further testing.
A positive self-test requires laboratory confirmatory testing to verify the initial result. Confirmatory testing ensures accuracy before any medical decisions are made.
Compared to blood-based self-tests, oral fluid-based tests generally have:
Higher sensitivity.
Lower specificity.
Slightly lower sensitivity.
No difference in accuracy.
Oral fluid tests are generally slightly less sensitive than blood-based tests due to lower antibody concentrations in saliva. Specificity remains similar.
For a finger-prick blood self-test, what is the recommended site for obtaining the sample?
Middle finger pad.
Tip of the index finger.
Palm.
Fingernail edge.
The side of the fingerpad, particularly the index finger, provides sufficient blood flow and minimizes discomfort. Avoiding the fingertip reduces pain.
If no control line appears on an HIV self-test, what should be concluded?
Negative result.
Positive result.
Test is invalid and should be redone.
Sample insufficient, but result valid.
Absence of a control line indicates a malfunction or error during testing. The test should be repeated with a new kit.
Which of the following best explains why confirmatory testing is important after a positive self-test?
To detect different HIV strains.
To verify results and rule out false positives.
To measure viral load.
To determine treatment protocol.
Confirmatory testing helps distinguish true positives from false positives. Laboratory assays offer higher accuracy before a diagnosis is made.
What is the safest way to dispose of lancets used in HIV self-testing?
Drop in kitchen waste.
Place in a rigid sharps container.
Wrap in paper and discard.
Throw in recycling bin.
Rigid sharps containers are designed to safely contain needles and lancets. This prevents accidental needle-stick injuries and biohazard exposure.
At what temperature should most HIV self-test kits be stored for optimal performance?
2 - 30°C (36 - 86°F).
Below 0°C.
Above 50°C.
Room temperature above 40°C.
Storing kits between 2 and 30°C maintains reagent stability and test accuracy. Extreme temperatures can degrade test components.
Why is timing critical during the reading phase of an HIV self-test?
To allow the test strip to dry out.
To ensure accurate interpretation within the specified window.
To calibrate the color.
To let the control line disappear.
Each test specifies a window in which results are valid; reading outside this window can alter line appearance. Accurate timing is essential for reliable interpretation.
When performing an HIV self-test, wearing gloves is:
Always required by kit instructions.
Discouraged due to contamination risk.
Unnecessary and not recommended.
Optional but recommended if blood exposure risk.
While some kits may not require gloves, wearing them is recommended when there is a risk of blood exposure to protect both the user and others. Gloves serve as a barrier against potential infection.
A test with 99% specificity is used in a low prevalence setting. How does this affect the positive predictive value?
It increases the positive predictive value.
It has no effect on positive predictive value.
It decreases positive predictive value leading to more false positives.
Positive predictive value equals specificity.
In low prevalence settings, even high specificity yields lower positive predictive value, increasing false-positive rates. Thus, many positive results may not indicate true infection.
Which describes a key reason confirmatory testing uses a different methodology than the initial self-test?
To reduce cost.
To provide a faster result.
To use a more sensitive and specific laboratory assay.
To avoid blood handling.
Confirmatory tests use laboratory assays like Western blot or PCR, which have higher sensitivity and specificity than rapid self-tests. Different methodologies reduce the chance of false results.
Which detection principle distinguishes a fourth-generation HIV test from a third-generation test in self-testing?
Detection of HIV RNA.
Detection of both p24 antigen and antibodies.
Detection of antibodies only.
Detection of CD4 cell count.
Fourth-generation tests identify both p24 antigen and antibodies, detecting HIV earlier than antibody-only tests. This reduces the window period compared to third-generation tests.
What is a crucial step to prevent cross-contamination when performing multiple HIV self-tests in succession?
Testing on the same surface without cleaning.
Using the same lancet for each test.
Clean the work area and change gloves between tests.
Store used kits nearby.
Cleaning surfaces and changing gloves prevents transfer of biological material between tests. These measures ensure that each test remains uncontaminated.
A faint test line appears within the valid reading window on an HIV self-test. What is the most appropriate interpretation?
Negative result.
Positive result, even if faint.
Invalid, must retest.
Control line failure.
Even a faint test line indicates the presence of HIV antibodies and should be interpreted as positive. Any visible test line within the reading window is considered a reactive result.
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Learning Outcomes

  1. Identify proper procedures for HIV self-testing.
  2. Interpret self-test outcomes accurately.
  3. Explain the significance of confirmatory testing.
  4. Compare various HIV self-testing methods.
  5. Apply safe handling and disposal practices.

Cheat Sheet

  1. Understand proper procedures for HIV self-testing - Mastering each step makes self-testing feel as easy as cooking your favorite snack. You'll learn how to collect samples safely and read the results like a detective uncovering clues. Correctly formatted link
  2. cdc.gov
  3. Interpret self-test outcomes accurately - Think of your test strip as a secret code that you get to crack! This point shows you how to read lines and symbols correctly so there's no confusion about what you see. Correctly formatted link
  4. cdc.gov
  5. Recognize the significance of confirmatory testing - A positive result is like finding an unexpected plot twist in a story - it means you need to head to a healthcare facility for the official follow-up. Confirmatory tests ensure you have the clear facts before making any decisions. Correctly formatted link
  6. cdc.gov
  7. Compare various HIV self-testing methods - From painless oral swabs to quick finger-prick tests, explore the pros and cons of each approach. This way, you can pick the method that fits your style - no lab coat required! Correctly formatted link
  8. cdc.gov
  9. Apply safe handling and disposal practices - Treat your used test materials like biohazard stars: wrap them up, seal them safely, and send them to their final resting place without worry. Proper disposal keeps you and your environment as clean as a whistle. Correctly formatted link
  10. cdc.gov
  11. Be aware of the window period - Imagine a "sneak peek" phase when the virus isn't yet visible - this is the window period. Knowing this helps you pick the right time to test, so you're not left wondering if you tested too early. Correctly formatted link
  12. cdc.gov
  13. Know the limitations of self-testing - Self-tests are like at-home quizzes: super convenient, but not a replacement for your final exam at a clinic. Learn when it's time to level up to professional testing if you're at ongoing risk. Correctly formatted link
  14. cdc.gov
  15. Understand the importance of linkage to care - Getting a positive result is just the opening chapter of your health journey. This study point emphasizes how vital it is to connect with healthcare services for the next steps and support. Correctly formatted link
  16. who.int
  17. Stay informed about approved testing kits - Only FDA-approved kits earn their gold star for accuracy and safety. This section guides you on spotting legit products so you always get reliable results. Correctly formatted link
  18. fda.gov
  19. Recognize the role of self-testing in public health - Every self-test you take plays a part in a bigger story: improving community health by making testing more accessible. Learn how your own effort can create a ripple effect of awareness and prevention. Correctly formatted link
  20. who.int
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