Chromatography
The number of moles of solute present in 1 kg of a solvent is called its
molality
molarity
normality
formality
Which of the following properties is not used as a basis for physical separation of analyte species in analytical chemistry?
mass
polarity
molar absorptivity
physical state
size
What is the main difference between HPLC and UPLC?
HPLC is reverse-phase whereas UPLC is normal-phase
UPLC employs smaller stationary-phase particle size
HPLC and UPLC employ different mobile phases
HPLC and UPLC employ different detection methods
HPLC operates at higher mobile phase pressures
Accuracy is defined as:
A measure of how often an experimental value can be repeated.
The closeness of a measured value to the real value.
The number of significant figures used in a measurement.
None of these
How many significant figures are present in the number 10,450?
three
four
five
none of these
Chromatography is used to:
Separate two or more compounds based on their polarities.
Separate two or more compounds based on their masses.
Separate two or more compounds based on how strongly they interact with other compounds.
All of these
None of the above
If I dilute 5 mL of 0.15 M NaCl to a final volume of 5 L, what’s the final concentration of NaCl?
If it takes 5 mL of 1.4 M NaOH to neutralize 150 mL of HCl with an unknown concentration, what was the original concentration of the acid?
0.467 M
0.047 M
0.014 M
none of these
What is the solid particles used in chromatography called?
The solid phase
The stationary phase
The column phase
The particular phase
What is the liquid used in chromatography called?
The pumped phase
The transparent phase
The mobile phase
The solution phase
What makes the liquid pass through the HPLC column?
The capillary forces
A pump
Gravity
Electricity
What is the function of the injector?
It injects the sample solution into the HPLC system
It injects energy into the column to facilitate the separation
It injects light into the detector
It injects liquid into the reservoir
Why should not mobile phases with pH above 8 be used with silica based columns?
The silica dissolves at high pH so that the column deteriorates
The mobile phase becomes flammable at high pH
The pressure becomes too high
The analytes degrade at high pH
Which organic modifiers are most common in Reversed Phase Chromatography
Isopropanol and toluene
Methylenechloride and chloroform
Methanol and Acetonitrile
Ether and acetone
Chromatographic retention is due to:
Different injection times by the autosampler
Adsorption of the analyte to the stationary phase
Differences in absorbance in the UV detector
Deviations in the flow from the pump
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